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quest graph ec50 calculator  (AAT Bioquest)

 
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    AAT Bioquest quest graph ec50 calculator
    Quest Graph Ec50 Calculator, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quest+graph+ec50+calculator/pmc11091963__pt4c00037_si_001-11-5-9?v=AAT+Bioquest
    Average 90 stars, based on 1 article reviews
    quest graph ec50 calculator - by Bioz Stars, 2026-07
    90/100 stars

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    A: Results of a screen of 2,400 FDA-approved drugs identified to transcriptionally activate (>1), inhibit (<1), or not affect (=1) MNRR1. Each circle represents one drug and the MNRR1 activator (Nitazoxanide (N)) has been highlighted in green. B: Equal numbers of HTR cells overexpressing the MNRR1-luciferase reporter were plated on a 96 well plate and treated with increasing amounts of nitazoxanide or vehicle (DMSO) for 24 h. <t>EC50</t> was calculated using the activation of MNRR1 reporter relative to vehicle treated cells using the Quest Graph(tm) EC50 Calculator. C: TNF transcript levels were measured in human placental cells. Actin was used as a housekeeping gene to normalize expression (n=4, for all figures, significance is indicated with * and represents p-value<0.05, ns is non-significant).
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    A: Results of a screen of 2,400 FDA-approved drugs identified to transcriptionally activate (>1), inhibit (<1), or not affect (=1) MNRR1. Each circle represents one drug and the MNRR1 activator (Nitazoxanide (N)) has been highlighted in green. B: Equal numbers of HTR cells overexpressing the MNRR1-luciferase reporter were plated on a 96 well plate and treated with increasing amounts of nitazoxanide or vehicle (DMSO) for 24 h. EC50 was calculated using the activation of MNRR1 reporter relative to vehicle treated cells using the Quest Graph(tm) EC50 Calculator. C: TNF transcript levels were measured in human placental cells. Actin was used as a housekeeping gene to normalize expression (n=4, for all figures, significance is indicated with * and represents p-value<0.05, ns is non-significant).

    Journal: bioRxiv

    Article Title: MNRR1 activation by nitazoxanide abrogates lipopolysaccharide-induced preterm birth in mice

    doi: 10.1101/2023.01.30.525762

    Figure Lengend Snippet: A: Results of a screen of 2,400 FDA-approved drugs identified to transcriptionally activate (>1), inhibit (<1), or not affect (=1) MNRR1. Each circle represents one drug and the MNRR1 activator (Nitazoxanide (N)) has been highlighted in green. B: Equal numbers of HTR cells overexpressing the MNRR1-luciferase reporter were plated on a 96 well plate and treated with increasing amounts of nitazoxanide or vehicle (DMSO) for 24 h. EC50 was calculated using the activation of MNRR1 reporter relative to vehicle treated cells using the Quest Graph(tm) EC50 Calculator. C: TNF transcript levels were measured in human placental cells. Actin was used as a housekeeping gene to normalize expression (n=4, for all figures, significance is indicated with * and represents p-value<0.05, ns is non-significant).

    Article Snippet: EC50 was calculated using Quest Graph(tm) EC50 Calculator ( AAT Bioquest, Inc ., https://www.aatbio.com/tools/ec50-calculator ).

    Techniques: Luciferase, Activation Assay, Expressing